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MolBreeding Biotech Ltd multiplex pcr primer panel design
The procedure of <t>target</t> <t>SNP-seq</t> in genotyping 261 varieties with 163 perfect SNP. Library construction of target SNP-seq included two round of <t>PCR.</t> The first round of PCR was to capture the target SNP locus through multiplex PCR. The second round of PCR aimed to add a unique barcode adaptor for each DNA sample. Finally, the constructed library was sequenced on Highseq X platform.
Multiplex Pcr Primer Panel Design, supplied by MolBreeding Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/multiplex pcr primer panel design/product/MolBreeding Biotech Ltd
Average 90 stars, based on 1 article reviews
multiplex pcr primer panel design - by Bioz Stars, 2026-06
90/100 stars

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Article Title: A new SNP genotyping technology Target SNP-seq and its application in genetic analysis of cucumber varieties

Journal: Scientific Reports

doi: 10.1038/s41598-020-62518-6

The procedure of target SNP-seq in genotyping 261 varieties with 163 perfect SNP. Library construction of target SNP-seq included two round of PCR. The first round of PCR was to capture the target SNP locus through multiplex PCR. The second round of PCR aimed to add a unique barcode adaptor for each DNA sample. Finally, the constructed library was sequenced on Highseq X platform.
Figure Legend Snippet: The procedure of target SNP-seq in genotyping 261 varieties with 163 perfect SNP. Library construction of target SNP-seq included two round of PCR. The first round of PCR was to capture the target SNP locus through multiplex PCR. The second round of PCR aimed to add a unique barcode adaptor for each DNA sample. Finally, the constructed library was sequenced on Highseq X platform.

Techniques Used: Multiplex Assay, Construct



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MolBreeding Biotech Ltd multiplex pcr primer panel design
The procedure of <t>target</t> <t>SNP-seq</t> in genotyping 261 varieties with 163 perfect SNP. Library construction of target SNP-seq included two round of <t>PCR.</t> The first round of PCR was to capture the target SNP locus through multiplex PCR. The second round of PCR aimed to add a unique barcode adaptor for each DNA sample. Finally, the constructed library was sequenced on Highseq X platform.
Multiplex Pcr Primer Panel Design, supplied by MolBreeding Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/multiplex pcr primer panel design/product/MolBreeding Biotech Ltd
Average 90 stars, based on 1 article reviews
multiplex pcr primer panel design - by Bioz Stars, 2026-06
90/100 stars
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The procedure of target SNP-seq in genotyping 261 varieties with 163 perfect SNP. Library construction of target SNP-seq included two round of PCR. The first round of PCR was to capture the target SNP locus through multiplex PCR. The second round of PCR aimed to add a unique barcode adaptor for each DNA sample. Finally, the constructed library was sequenced on Highseq X platform.

Journal: Scientific Reports

Article Title: A new SNP genotyping technology Target SNP-seq and its application in genetic analysis of cucumber varieties

doi: 10.1038/s41598-020-62518-6

Figure Lengend Snippet: The procedure of target SNP-seq in genotyping 261 varieties with 163 perfect SNP. Library construction of target SNP-seq included two round of PCR. The first round of PCR was to capture the target SNP locus through multiplex PCR. The second round of PCR aimed to add a unique barcode adaptor for each DNA sample. Finally, the constructed library was sequenced on Highseq X platform.

Article Snippet: Finally, all perfect SNP loci were sent to the Molbreeding Biotechnology Company (Shijiazhuang, China) for multiplex PCR primer panel design.

Techniques: Multiplex Assay, Construct